gfap 1 Search Results


anti-gfap 1:1000 dilution  (Merck KGaA)
90
Merck KGaA anti-gfap 1:1000 dilution
Anti Gfap 1:1000 Dilution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/anti+gfap+1+1000+dilution/pmc10216629-73-18-21
Average 90 stars, based on 1 article reviews
anti-gfap 1:1000 dilution - by Bioz Stars, 2026-09
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primary antibodies (anti-gfap 1:100  (Diagnostic BioSystems)
90
Diagnostic BioSystems primary antibodies (anti-gfap 1:100
Primary Antibodies (Anti Gfap 1:100, supplied by Diagnostic BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/primary+antibodies++anti+gfap+1+100/pmc11706855-89-29-31
Average 90 stars, based on 1 article reviews
primary antibodies (anti-gfap 1:100 - by Bioz Stars, 2026-09
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rabbit anti-gfap (1:200)  (AnaSpec)
90
AnaSpec rabbit anti-gfap (1:200)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Rabbit Anti Gfap (1:200), supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/rabbit+anti+gfap++1+200+/pmc03578497-174-29-48
Average 90 stars, based on 1 article reviews
rabbit anti-gfap (1:200) - by Bioz Stars, 2026-09
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anti-chicken-gfap 1:1000  (Nordic BioSite)
90
Nordic BioSite anti-chicken-gfap 1:1000
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Anti Chicken Gfap 1:1000, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/mouse+anti+glial+fibrillary+acidic+protein++gfap++1+1000/pm22059637-76-17-19
Average 90 stars, based on 1 article reviews
anti-chicken-gfap 1:1000 - by Bioz Stars, 2026-09
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anti-glial fibrillary acid protein (gfap)  (Cymbus Biotechnology)
90
Cymbus Biotechnology anti-glial fibrillary acid protein (gfap)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Anti Glial Fibrillary Acid Protein (Gfap), supplied by Cymbus Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/anti+glial+fibrillary+acid+protein++gfap++1+200+/pmc03866165-92-34-41
Average 90 stars, based on 1 article reviews
anti-glial fibrillary acid protein (gfap) - by Bioz Stars, 2026-09
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rabbit anti-glial fibrillary acidic protein (gfap) (1:100)  (Becton Dickinson)
90
Becton Dickinson rabbit anti-glial fibrillary acidic protein (gfap) (1:100)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Rabbit Anti Glial Fibrillary Acidic Protein (Gfap) (1:100), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/rabbit+anti+glial+fibrillary+acidic+protein++gfap+++1+100+/pmc02475778-112-11-6
Average 90 stars, based on 1 article reviews
rabbit anti-glial fibrillary acidic protein (gfap) (1:100) - by Bioz Stars, 2026-09
90/100 stars
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glial fibrillary acidic protein (gfap) (×1,000)  (MBL Life science)
90
MBL Life science glial fibrillary acidic protein (gfap) (×1,000)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Glial Fibrillary Acidic Protein (Gfap) (×1,000), supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/glial+fibrillary+acidic+protein++gfap+++%C3%971+000++antibody/pm26616272-96-29-35
Average 90 stars, based on 1 article reviews
glial fibrillary acidic protein (gfap) (×1,000) - by Bioz Stars, 2026-09
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polyclonal rabbit anti-glial fibrillary acid protein ab (gfap, 1:100; icn pharmaceutical, frankfurt, germany)  (ICN Pharmaceuticals)
90
ICN Pharmaceuticals polyclonal rabbit anti-glial fibrillary acid protein ab (gfap, 1:100; icn pharmaceutical, frankfurt, germany)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Polyclonal Rabbit Anti Glial Fibrillary Acid Protein Ab (Gfap, 1:100; Icn Pharmaceutical, Frankfurt, Germany), supplied by ICN Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/polyclonal+rabbit+anti+glial+fibrillary+acid+protein+ab++gfap++1+100++icn+pharmaceutical++frankfurt++germany+/10__1523_slash_JNEUROSCI__23___07___02517__2003_ascii32_-83-46-55
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-glial fibrillary acid protein ab (gfap, 1:100; icn pharmaceutical, frankfurt, germany) - by Bioz Stars, 2026-09
90/100 stars
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antibody solution of anti-gfap (1:100)  (Shandon Lipshaw)
90
Shandon Lipshaw antibody solution of anti-gfap (1:100)
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
Antibody Solution Of Anti Gfap (1:100), supplied by Shandon Lipshaw, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/antibody+solution+of+anti+gfap++1+100+/10__1523_slash_jneurosci__16___24___08005__1996-48-30-32
Average 90 stars, based on 1 article reviews
antibody solution of anti-gfap (1:100) - by Bioz Stars, 2026-09
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21_03_21_100_2017_12_aldh1l1_1; 21_05_11_100_2017_12_gfap_1; 21_05_11_100_2017_29_aldh1l1_1  (3DHistech ltd)
90
3DHistech ltd 21_03_21_100_2017_12_aldh1l1_1; 21_05_11_100_2017_12_gfap_1; 21_05_11_100_2017_29_aldh1l1_1
DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for <t>GFAP</t> showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity <t>to</t> <t>PCNA,</t> which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.
21 03 21 100 2017 12 Aldh1l1 1; 21 05 11 100 2017 12 Gfap 1; 21 05 11 100 2017 29 Aldh1l1 1, supplied by 3DHistech ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfap+1/21+04+20+104+12+a+aldh1l1+1++21+05+11+104+12+i+gfap+1++21+04+20+np104+12+a+gfap+1++21+05+11+104+12+i+aldh1l+1/pm38242926-53-122-128
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21_03_21_100_2017_12_aldh1l1_1; 21_05_11_100_2017_12_gfap_1; 21_05_11_100_2017_29_aldh1l1_1 - by Bioz Stars, 2026-09
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Image Search Results


DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for GFAP showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity to PCNA, which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.

Journal: Neuro-Oncology

Article Title: Glioma is formed by active Akt1 alone and promoted by active Rac1 in transgenic zebrafish

doi: 10.1093/neuonc/nos387

Figure Lengend Snippet: DAAkt1-induced gliomas. (A) Gross and histologic findings. (a,b) Glioma-bearing 3-month-old Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish with bent body and visible bumps at the head, showing strong GFP expression on merged bright and fluorescence images (inlets). (c) Hematoxylin and eosin stains of cerebellum in control zebrafish. (d–i) Hematoxylin and eosin stains of gliomas. (d and e) Small tumors (boundaries by red arrowheads) at periventricular area showing invasion into 4th ventricle. (f) A large glioma replacing almost the whole cerebellum. (g) A large glioma showing invasion into the midbrain. (h) A high-grade glioma showing increased vascularity (black arrowhead). (i) A high-grade glioma showing necrosis (red arrowhead). (j) IHC for GFAP showing robust expression. (k) IHC for Akt1 on a small tumor invading 4th ventricle showing expression at tumor. (l) ISH for DAAkt1 showing stronger expression at the hypercellular area. (B) Increased proliferation in DAAkt1-induced gliomas. IHC indicates highly frequent positivity to PCNA, which is even higher in high-grade tumor. Tumors occasionally show positive staining to PHH3, a mitotic marker. BrdU labeling also reveals frequently positive cells. (C) Proliferation analyses in the pre-neoplastic cerebellum of 2-month-old zebrafish. Reactivity at the intestinal cells is used as an internal positive control (Inlets). Cells at the ventricular lining and the intermediate layer frequently reveal immunoreactivity to proliferative markers. The non–tumor-bearing cerebellum of DAAkt1-expressing zebrafish is not associated with increased proliferation. Average numbers of positive cells counted at the periventricular are 4.5 ± 2.2 and 4.7 ± 2.6 for PHH3 and 18.7 ± 6.8 and 19.9 ± 6.9 for BrdU in Ptf1aGal4/UASGFP and Ptf1aGal4/UASGFP-UASDAAkt1 zebrafish, respectively, which are not significantly different. Bars, 50 μm. (D) Activation of Akt downstream components in nonneoplastic DAAkt1-expressing cerebellum. IHC analyses reveal slightly increased expression of phospho-mTOR, -RS6K, and -4EBP1 in cells at the ventricular and periventricular zone.

Article Snippet: 29 , 34 Primary antibodies were as follows: rabbit anti-Akt1 (1:500), mouse anti-Rac1 (1:500), mouse anti-proliferating cell nuclear antigen (PCNA) (1:2000), and rabbit anti-pancadherin (1:500) from Abcam (Cambridge, MA); rabbit anti-GFAP (1:200), rabbit anti-snail1a (1:500), rabbit anti-β-catenin1 (1:200), rabbit anti-caspase a (1:500), and rabbit anti-caspase b (1:500) from Anaspec (Fremont, CA, USA); and rabbit anti-phospho-histoneH3 (pHH3; 1:200), rabbit anti-phospho-mTOR (1:200), rabbit anti-phospho-4EBP1 (1:200), and rabbit anti-phospho-RS6K (1:200) from Cell Signaling (Danvers, MA).

Techniques: Expressing, Fluorescence, Staining, Marker, Labeling, Positive Control, Activation Assay